Antibodies have been used extensively over the past few decades as an extremely powerful and useful tool to detect, quantify, characterize, purify, and purify molecules of interest. Their utility is based on their ability to selectively bind an epitope from carbohydrate, protein or nucleic acids.
Research uses two types of primary antibodies: monoclonal and polyclonal. Both monoclonal and polyclonal antibodies play an important role in the immune response and can be used to address different research questions. There are many differences in the production methods that have led to monoclonal and multiclonal antibodies having different characteristics.
This article will provide an overview of their production process, distinguishing properties, and roles in research, diagnostics and therapeutics.
Polyclonal antibodies (IgGs) are heterogeneous combinations of immunoglobulins that recognize and bind to multiple epitopes within the same antigen.
A low dose of an antigen (proteins or synthetic peptides), is used to produce polyclonal antibodies. To stimulate the production of antibodies, inject different types of antigens into animals. The animal receives a secondary, or even tertiary booster vaccination of the same antigen after several days to increase the titers.
You can either obtain polyclonal antibodies directly from the serum, or you can purify the solution to remove other serum proteins. This will limit cross-reactivity.
Monoclonal antibodies, which are ex-vivo from a single B-cell in an animal, are not produced in vitro. Stable hybrid B cell clones were created from one parent cell, resulting in an identical population of monovalent IgG that targets a specific epitope of each antigen.
The production process starts with multiple immunizations of the animal with the desired antibody. After several weeks, B-cells from the animal’s spleen are removed and immortalized with hybridoma cell fusion (spleen cells cannot grow in culture). Then the antibody screening is performed.
Hybridomas can be grown continuously in culture and produce antibodies. This allows for unlimited production of monoclonal antibodies.
Choosing an Antibody
Monoclonal antibodies’ true potential lies in their monospecificity and homogeneity as well as their consistency. These characteristics strongly suggest their use in clinical settings and affinity purification. Monoclonal antibodies with the desired specificity can be difficult to produce, are expensive and take a lot of time.
Monoclonal antibodies can be used in a variety of ways, including:
Therapeutic drug development
The advantages of polyclonal antibody are often more beneficial than monoclonal ones for general research purposes. Monoclonal antibodies are more difficult to make than polyclonal antibodies. They can be produced much faster, for a lower price, and require less technical skill.
The polyclonal antibody is suitable for many applications including:
ELISA, WB, microarray assays, IHC, etc.
IP or ChIP.
Custom Rabbit Polyclonal Antibody Production Service
There are many host species that you can choose from to generate your antibodies. For producing polyclonal antibody, the most common host is rabbit. Because rabbit has a wider range of antigen classes than other hosts, it is the most commonly used host. It also produces the highest yields in the shortest time (1-5 mg for purification of a 20-mL serum).
The production of antibodies is complex and requires a high level of technical knowledge. There are many reagents available that can be used to solve problems quickly and reduce costs, but these products may not always meet the needs of specific research projects.
If you are looking for additional security and assurance in your scientific successes, custom rabbit polyclonal antibodies production and development can be a good option.
Boster Bio is a world-renowned antibody manufacturer and offers a cost-effective, reliable, and flexible custom rabbit polyclonal antibody production service. This will help you manage your polyclonal antibodies development project, from antigenic selection through to the delivery of affinity purified antibodies. Boster’s custom-made rabbit polyclonal antibody service platform is capable of producing high-quality custom bunny polyclonal antibodies that can be used in downstream applications.